Using Silicon Polymer Impression Technique and Scanning Electron Microscopy to Measure Stomatal Aperture, Morphology, and Density.

Abstract

The number of stomata on leaves can be affected by intrinsic development programming and various environmental factors, in addition the control of stomatal apertures is extremely important for the plant stress response. In response to elevated temperatures, transpiration occurs through the stomatal apertures, allowing the leaf to cool through water evaporation. As such, monitoring of stomata behavior to elevated temperatures remains as an important area of research. The protocol allows analysis of stomatal aperture, morphology, and density through a non-destructive imprint of Arabidopsis thaliana leaf surface. Stomatal counts were performed and observed under a scanning electron microscope.

Code and data availability

This is a Bio-protocol methods article describing a silicon impression/SEM technique for measuring stomatal aperture, morphology, and density. The only software mentioned is ImageJ, a generic third-party image analysis library cited with its standard URL, not an authors' deposited analysis code, dataset, image set, or训

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