RTIP: A FULLY AUTOMATED ROOT TIP TRACKER FOR MEASURING PLANT GROWTH WITH INTERMITTENT PERTURBATIONS.

Abstract

RTip is a tool to quantify plant root growth velocity using high resolution microscopy image sequences at sub-pixel accuracy. The fully automated RTip tracker is designed for high-throughput analysis of plant phenotyping experiments with episodic perturbations. RTip is able to auto-skip past these manual intervention perturbation activity, i.e. when the root tip is not under the microscope, image is distorted or blurred. RTip provides the most accurate root growth velocity results with the lowest variance ( i.e. localization jitter) compared to six tracking algorithms including the top performing unsupervised Discriminative Correlation Filter Tracker and the Deeper and Wider Siamese Network. RTip is the only tracker that is able to automatically detect and recover from (occlusion-like) varying duration perturbation events.

Code and data availability

The article describes the RTip root tip tracker and its evaluation on two root image sequences, but contains no data availability statement, no public repository link, no code deposit, and no supplementary asset references. The root tip image sequences, ground truth annotations, and RTip implementation are not stated (

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