In Vivo Spectroscopy.

Abstract

In vivo spectroscopy is used to directly assay phytochromes in intact plant material. The method is depending on the photoreversibility of phytochromes displaying light induced absorbance changes in response to actinic irradiation. Dual-wavelength ratio spectrophotometers (ratiospects) are the instruments successfully used for assaying phytochromes in highly scattering plant material. In the present chapter I describe the general instrument setup of an automated ratiospect and explain the measuring procedure and data calculation required to determine the total amount of photoreversible phytochromes in a sample as well as the proportion of phytochrome present in the Pfr conformation.

Code and data availability

公開本文の所在を確認できませんでした。非公開または購読が必要な可能性があります。

No evidence-backed public reproduction asset is currently recorded.