Non-invasive hydrodynamic imaging in plant roots at cellular resolution

Abstract

Abstract A key impediment to studying water-related mechanisms in plants is the inability to non-invasively image water fluxes in cells at high temporal and spatial resolution. Here, we report that Raman microspectroscopy, complemented by hydrodynamic modelling, can achieve this goal - monitoring hydrodynamics within living root tissues at cell- and sub-second-scale resolutions. Raman imaging of water-transporting xylem vessels in Arabidopsis thaliana mutant roots reveals faster xylem water transport in endodermal diffusion barrier mutants. Furthermore, transverse line scans across the root suggest water transported via the root xylem does not re-enter outer root tissues nor the surrounding soil when en-route to shoot tissues if endodermal diffusion barriers are intact, thereby separating ‘two water worlds’.

Code and data availability

The paper's MECHA 3D solute advection-diffusion model and MATLAB inverse modeling code are openly available on GitHub under a GPL.2 license. Other deposits (Raman data, hydraulic conductivity data, custom RMS analysis code on FigShare) exist but their URLs are not in the allowed list, so only the GitHub code asset is a