Singlet Oxygen and Protochlorophyllide Detection in Arabidopsis thaliana.

Abstract

Since the recognition of the reactive oxygen species singlet oxygen ( 1 O 2 ) as a versatile signal that induces various stress responses, the mechanisms underlying 1 O 2 -induced signaling transduction pathways have become the subject of much current research. This in turn highlights the need for reliable detection methods for 1 O 2 . Here we describe a protocol for the detection of 1 O 2 using a commercially available fluorescent probe (Singlet Oxygen Sensor Green) and provide a simple method for direct visualization and quantification of the 1 O 2 -evolving photosensitizer protochlorophyllide in the Arabidopsis fluorescent mutant.

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