Fluorescence visualization of cellulose and pectin in the primary plant cell wall

Abstract

Summary Plant cell walls constitute the extracellular matrix surrounding plant cells and are composed mainly of polysaccharides. The chemical makeup of the primary plant cell wall, and specifically, the abundance, localization and arrangement of the constituting polysaccharides are intimately linked with growth, morphogenesis and differentiation in plant cells. Visualization of the cell wall components is, therefore, a crucial tool in plant cell developmental studies. In this technical update, we present protocols for fluorescence visualization of cellulose and pectin in selected plant tissues and illustrate examples of some of the available labels that hold promise for live imaging of plant cell wall expansion and morphogenesis.

Code and data availability

公開本文の所在を確認できませんでした。非公開または購読が必要な可能性があります。

No evidence-backed public reproduction asset is currently recorded.