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Automated 3D Phenotyping of Maize Plants: Stereo Matching Guided by Deep Learning

Juan Zapata-Londoño · Juan Botero-Valencia · Ítalo A. Torres · Erick Reyes-Vera · Ruber Hernández-García

Agriculture · 12 Dec 2025 · 10.3390/agriculture15242573

Abstract

Automated three-dimensional plant phenotyping is an essential tool for non-destructive analysis of plant growth and structure. This paper presents a low-cost system based on stereo vision for depth estimation and morphological characterization of maize plants. The system incorporates an automatic detection stage for the object of interest using deep learning techniques to delimit the region of interest (ROI) corresponding to the plant. The Semi-Global Block Matching (SGBM) algorithm is applied to the detected region to compute the disparity map and generate a partial three-dimensional representation of the plant structure. The ROI delimitation restricts the disparity calculation to the plant area, reducing processing of the background and optimizing computational resource use. The deep learning-based detection stage maintains stable foliage identification even under varying lighting conditions and shadowing, ensuring consistent depth data across different experimental conditions. Overall, the proposed system integrates detection and disparity estimation into an efficient processing flow, providing an accessible alternative for automated three-dimensional phenotyping in agricultural environments.

Code and data availability

The paper's Data Availability Statement openly deposits the original study data (the 544 stereo RGB maize images and related phenotyping data) on OSF at a DOI, which is a paper-specific, publicly actionable asset. No author analysis code repository is explicitly stated.

Datasetpublic

Data Availability Statement: The original data presented in the study are openly available in OSF at https://doi.org/10.17605/OSF.IO/MN6P9.

Open resource ↗OSF · 10.17605/OSF.IO/MN6P9 · pdf-page:18 lines:1-59