An improved high-throughput method for assessing the impact of bioformulation on plant parameters: Case study with pigeonpea seeds

Abstract

Pigeonpea is an important legume cultivated in India, owing to its high nutritive and protein value. Various eco-friendly and green strategies have been proposed by researchers for enhancing the productivity of this pulse crop. In the present study, different bioformulations were prepared from plant growth promoting (PGP) bacterial strains and coated on pigeonpea seeds for monitoring growth enhancement of plantlets under in vitro conditions. For performing initial in vitro experiments with efficient bioinoculants, the conventional methods employ either tubes or Petri dishes. Despite delivering satisfactory results under laboratory conditions these methods suffer from inherent limitations of being cumbersome, difficult, and error-prone when multiple samples are handled simultaneously. The present study aimed to develop a high-throughput method for recording plant parameters of more than ninety seeds simultaneously, thus facilitating the process. The study opens up avenues of faster assessment to answer a range of scientific questions related to plant-microbe interactions and beyond.

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