In Vivo Detection of IAA Using IAA Nanosensor.

Abstract

Indole-3-acetic acid (IAA) is an important auxin phytohormone that regulates development, directional growth, and stress responses in plants. Here, we provide a detailed method and protocol for use of a corona phase molecular recognition-based IAA nanosensor that allows for direct and real-time measurement of IAA in plants. The near-infrared signal of the IAA nanosensor also allows it to be used in green tissues, as it is not affected by chlorophyll content. Furthermore, the IAA nanosensor can be easily applied across various plant species and all tissues as the sensor is not genetically encoded.

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