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Quantitative monitoring of Arabidopsis thaliana growth and development using high-throughput plant phenotyping

Daniel Arend · Matthias Lange · Jean-Michel Pape · Kathleen Weigelt-Fischer · Fernando Arana-Ceballos · Ingo Mücke · Christian Klukas · Thomas Altmann · Uwe Scholz · Astrid Junker

Scientific Data · 16 Aug 2016 · 10.1038/sdata.2016.55

Abstract

Abstract With the implementation of novel automated, high throughput methods and facilities in the last years, plant phenomics has developed into a highly interdisciplinary research domain integrating biology, engineering and bioinformatics. Here we present a dataset of a non-invasive high throughput plant phenotyping experiment, which uses image- and image analysis- based approaches to monitor the growth and development of 484 Arabidopsis thaliana plants (thale cress). The result is a comprehensive dataset of images and extracted phenotypical features. Such datasets require detailed documentation, standardized description of experimental metadata as well as sustainable data storage and publication in order to ensure the reproducibility of experiments, data reuse and comparability among the scientific community. Therefore the here presented dataset has been annotated using the standardized ISA-Tab format and considering the recently published recommendations for the semantical description of plant phenotyping experiments.

Code and data availability

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Codepublic

The IAP software is an open-source project and is available at https://github.com/OpenImageAnalysisGroup/IAP. For the presented analysis version 2.0.2 was used.

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