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Quantification of Starch in Guard Cells of Arabidopsis thaliana .

Flütsch S, Distefano L, Santelia D.

Bio-protocol · 1 Jul 2018 · 10.21769/bioprotoc.2920

Abstract

In this protocol, we describe how to quantify starch in guard cells of Arabidopsis thaliana using the fluorophore propidium iodide and confocal laser scanning microscopy. This simple method enables monitoring, with unprecedented resolution, the dynamics of starch in guard cells.

Code and data availability

This is a methods/protocol article describing guard cell starch quantification via propidium iodide staining and confocal imaging. The only software mentioned is ImageJ, a generic public analysis tool, not an authors' paper-specific asset; no phenotype datasets, images, code repositories, models, or supplements with a

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