Enhanced abscisic acid detection via SERS-active single crystal MAPbCl 3 nanofiber-based Self-CoAptaNano (SCAN) substrate.

Abstract

The development of novel SERS-active MAPbCl 3 nanofibers substrate offers a rapid, sensitive, and label-free method for critical stress phytohormone abscisic acid (ABA) detection as compared to conventional methods. Aptamers can act as a specific molecular recognition element that bring ABA molecules closer to the SERS-active MAPbCl 3 nanofiber surface, leading to a stronger localized electromagnetic field enhancement. The stable cross dimerized self-complementary (CDSC) aptamer configuration has the lowest Gibbs free energy (ΔG) of -9.75 kcal/mol according to thermodynamics. Bioinformatic analysis of aptamer using OligoAnalyzer® tool offered the thermodynamic properties and functional stability of the aptamer sequence designed to target the LOC109791758 gene encoding the Glycine-Rich Cell Wall Protein (GRCWP) in Cajanus cajan. This facilitated to develop the novel SERS-active single crystal MAPbCl 3 nanofiber-based Self-CoAptaNano (SCAN) substrate by improving the target molecule binding and sensitivity even at low concentrations. Material's structure and properties were characterized by using SEM, UV-Visible, and SERS in this study. The currently developed MAPbCl 3 nanofiber-based SCAN substrate for ABA detection resulted in better LOD of 1.17 × 10 -12 M for SERS and 2.14 × 10 -9 M for FLI as compared to previously developed substrates. Moreover, the EF was recorded as 1.08 × 10 7 M with the recovery rate close to 100 % and RSD of 3.24 % under SERS and 4.13 % under fluorescence exposure in complex matrices for ABA in real plant samples. The adaptability of MAPbCl 3 nanofiber-based SCAN as a substrate for aptamer-specific analysis via SERS can underscore their immense potential for broader applications in analytical chemistry and biotechnology.

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