Multimodal Analysis of Phytoalexin Synthesis in Arabidopsis by Mass Spectrometry Imaging and Fluorescent Microscopy.

Abstract

Phytoalexins are plant secondary antimicrobial compounds that are rapidly and locally accumulated de novo upon pathogen attacks. They are strongly correlated with disease resistance; therefore, the timing and the location of their synthesis and accumulation have been explored transcriptionally and metabolically using various means separately. In this chapter, by focusing on the Arabidopsis camalexin (CA), we describe protocols for multimodal in situ detection of CA and elemental distribution, as well as the transcriptionally active region of its synthesis gene PHYTOALEXIN DEFICIENT 3 (PAD3) within the same leaf sample challenged with a pathogen.

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