Visualization of cortical microtubule networks in plant cells by live imaging and immunostaining.

Abstract

Cortical microtubules (CMTs) play pivotal roles during plant cell growth and division. The organization of CMTs undergoes important changes during different cellular and developmental processes. Here, we describe two methods for the visualization of CMT organization in plant cells using confocal laser scanning microscopy. CMT networks in the outer tissue layers can be directly visualized by live imaging of a fluorescent reporter line, and a protocol combining sectioning and immunostaining is applied for visualization of CMTs throughout tissues. For complete details on the use and execution of this protocol, please refer to Zhao et al. (2020).

Code and data availability

This is a methods/protocol paper for visualizing cortical microtubules. It explicitly states 'This study did not generate unique datasets or code,' and no public phenotype datasets, images, analysis code, or trained models are deposited. Referenced tools (Fiji/FibrilTool, MorphoGraphX) are generic third-party libraries

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