Visualization of Actin Organization and Quantification in Fixed Arabidopsis Pollen Grains and Tubes.

Abstract

Although it is widely accepted that actin plays an important role in regulating pollen germination and pollen tube growth, how actin exactly performs functions remains incompletely understood. As the function of actin is dictated by its spatial organization, it is the key to reveal how exactly actin distributes in space in pollen cells. Here we describe the protocol of revealing and quantifying the spatial organization of actin using fluorescent phalloidin-staining in fixed Arabidopsis pollen grains and pollen tubes. We also introduce the method of assessing the stability and/or turnover rate of actin filaments in pollen cells using the treatment of latrunculin B.

Code and data availability

This is a Bio-protocol methods article describing phalloidin staining and ImageJ/R-based quantification of actin in Arabidopsis pollen. No public phenotype datasets, author-deposited images, analysis code, or trained models are mentioned. ImageJ and R are cited only as generic third-party tools, not paper-specific code

No evidence-backed public reproduction asset is currently recorded.